IFN-alpha 2b human

General description
The gene IFN-α2b (interferon α 2b) is mapped to human chromosome 9. The encoded protein is a type 1 interferon and a glycoprotein. It belongs to the class of cytokines. Human IFN-α2b is a 19.3kDa protein containing 165 amino acid residues.[1][2] Structurally, IFN-α2b is O-glycosylated at the threonine residue at position 106.[3] Application
IFN-α2b human has been used:
as an immunotherapeutic agent to test its antiproliferative and proapoptotic effects in RCC cell lines[4] to stimulate peripheral blood mononuclear cell (PBMC) prior to RNA isolation[5] to stimulate H4 cells before immunoprecipitation[6] to study role of USP18 (ubiquitin specific peptidase 18)-mediation deISGylation in controlling cellular autophagy upon treatment with type I interferons[6] Biochem/physiol Actions
IFN-α2b (interferon α 2b) is a lymphoid factor that possesses potent anti-viral activity. It mainly interacts with cell surface-specific receptors. This interaction is responsible for anti-pathogenic response in cells. IFN-α2b also exhibits anti-tumor activities. Humans with brain tumor were shown to have mutations in this gene.[1][2] IFN-α2b induces the secretion of cytokine IFN-γ in T cells.[7] The hydrogels embedded with IFN-α2b exploits this functionality in gastric cancer cells.[7] Recombinant IFN-α types are effective against Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in in vitro studies.[8] Physical form
Lyophilized from 5 mM sodium phosphate, pH 7.4.
Preparation Note
Reconstitute in water to a concentration of 1 mg/mL. This solution can then be diluted into other aqueous buffers. Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.