IgM from bovine serum

General description
IgM antibodies are present as pentamers in the serum and are produced in response to antigens[1] IgM from bovine serum is purified from normal bovine serum by precipitation and gel filtration techniques.
IgM is a highly conserved antibody in vertebrates and is expressed early during immune response.[2] It exists as a pentamer and is secreted by peritoneal B cells.[3] Application
IgM from bovine serum has been used for screening the lipopolysaccharide levels in Limulus amoebocyte lysate (LAL) assay.[4] IgM has been used in flow cytometry assay for detecting FcγRIV expression and immunoglobulin binding.[5] Purified bovine IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents.
Biochem/physiol Actions
IgM plays a key role in engulfing of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections.[3] IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules.[6] Physical form
Solution in 0.05 M Tris-HCl, 0.15 M sodium chloride and 0.01 M glycine, pH 7.4, containing 15 mM sodium azide
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