IL-1β human

General description
Interleukin-1β (IL1B) is produced by activated macrophages.[1] It is synthesized as pro-IL-1β which is changed to active form IL-1β with the help of pro-inflammatory protease caspase-1.[2] IL-1β belongs to the IL-1 gene family.[3] It is a proinflammatory cytokine.[4] The gene is mapped to human chromosome 2q14.[5] In comparison with the Escherichia coli expressed protein, IL-1β is 50% more potent in promoting human CD4+ T cell differentiation into Th17 (T helper) cells. This cytokine is produced in a serum free, chemically defined media.
Application
IL-1β (IL1B) has been used to check the expression of NGAL (neutrophil gelatinase-associated lipocalin) in epithelial cells after stimulation with proinflammatory cytokines.[6] It has been used to check anti-inflammatory activities of N-Acetylglucosamine in human chondrocytes.[7] It has been used to create inflammatory endothelium in human coronary artery endothelial cells.[8] Biochem/physiol Actions
IL-1β (IL1B) stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation and fibroblast growth factor activity.[1] IL-1β proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.[9][10] IL-1β is present in chronic inflamed tissues and also in tissues going for fibrogenesis.[3] It brings neutrophil and macrophage to the site of infection.[11] Mutations in IL-1β gene are linked with renal manifestations and renal sequelae in Henoch-Schönlein purpura.[12] It is also associated with destruction of β-cell in type 1 diabetes.[13] Physical form
Lyophilized from a PBS solution.
Preparation Note
Centrifuge the vial prior to opening.
Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.