English
General description
Keratinocyte Growth Factor(KGF) is a 18.9 kDa growth factor, spanning 163 amino acids,[1] belongs to the fibroblast growth factor family. It includes a secretory signal sequence and shares 39% identity with FGF-Basic. It is expressed in a number of stromal fibroblast cell lines[2], mesenchymal cells[3] but absent from normal glial cells and epithelial cell lines.
Application
Keratinocyte Growth Factor human has been used:
as a component of epithelial growth medium[4]
as a positive control for antigen to determine the activity of the antibody[5]
to analyze the influence of KGF associated with thermo-sterilized carboxymethyl chitosan (NOCCts) in the reduction of pericardial adhesions[6]
Biochem/physiol Actions
Keratinocyte Growth Factor(KGF) stimulates proliferation and differentiation of keratinocytes and other cells in the epithelium.[7] It induces chemotaxis of microvascular endothelial cells and neovascularization of rat cornea. It stimulates the hyaluronic acid synthesis in proliferating and migrating basal cells.[2] KGF contributes to the survival of cells under stress conditions.[3] KGF is highly expressed in injured and inflamed epithelial cells, thereby, promotes wound healing.[3]
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.
Analysis Note
Determined by a cell proliferation assay using 4MBr-5 cells.
