English
General description
L-15 Medium (Leibovitz) was initially prepared to be used in carbon dioxide (CO2) free systems, which involves sodium bicarbonate. The complement of free base amino acids, salts, and galactose buffers it. This aids the maintenance of physiological pH control.[1]
Application
L-15 Medium (Leibovitz) has been used:
in protein incorporation for incubation of high blastula embryos and to avoid the abnormal development of embryos[2]
as a supplement in sterile cell media for the dissection and isolation of kidney cells[3]
as a component of culture buffer for the tissue culture of Newt lung cells[4]
Preparation Note
Supplement with 0.3 g/L L-glutamine.
