General description
L-15 Medium (Leibovitz) is formulated for use in carbon dioxide (CO2) free systems requiring sodium bicarbonate supplements. It supports established cell lines, such as human larynx epidermoid cells (HEp-2) and Rhesus monkey kidney epithelial cells (LLC-MK2), as well as primary explants of embryonic and adult human tissue. L-15 medium can also be used for culturing viruses.
Application
L-15 Medium (Leibovitz) has been used:
to study the toxicity of silver nanoparticles (AgNPs) with primary cultures of Eisenia fetida coelomocytes[1]
to culture crab hepatopancreas and ovary tissues[2]
as a component of the dissociation medium for infusion of the pancreas of mice[3]
Preparation Note
Formulated to contain 13.8 grams of powder per liter of medium.

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