English
General description
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Application
Medium 199 has been used:
to test and determine the pH sensitivity of the sensors in a complex environment
as a culture medium during oocyte harvesting
as a component of Leishmania donovani (LdBOB) promastigote media for culturing L. donovani
to culture visceral adipose tissue explants
Preparation Note
Formulated to contain 10.6 grams of powder per liter of medium.
Supplement with 0.35 g/L sodium bicarbonate.
