English
General description
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Application
Medium 199 has been used:
to culture the primary human umbilical vein endothelial cells (HUVECs) for use in cell viability assay
to flush the uterine horn of gilts to recover day 12 conceptuses from uteri
to culture the peritoneal cells and immortalized human mesothelial cells (MeT-5A and ATCC-CRL-9444)
Preparation Note
Formulated to contain 9.4 grams of powder per liter of medium.
Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate.
