English
General description
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Application
Medium 199 has been used to:
maintain the cumulus-oocyte complex (COC) before maturation and for culturing of these oocytes for in vitro maturation[1]
to rinse the aspiration line during the collection of the ovum from German Holstein cows[2]
to culture cardiac endothelial cells from rats[3]
Preparation Note
Formulated to contain 14.7 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.
