English
General description
Minimum Essential Medium Eagle contains 19 amino acids. The essential amino acids and the non-essential amino acids: L-ala, L-asn, L-asp, L-glu, L-gly, L-pro, and L-ser. This medium is preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium. It is suitable for a wide variety of cells grown in monolayers.
Application
Minimum Essential Medium Eagle has been used:
to culture endometrial adenocarcinoma Ishikawa cells for bisphenol studies
to culture MCF-7 cells for experimental treatments with hormone
to maintain zebrafish pineal glands for action spectrum analysis
Features and Benefits
Contains high concentrations of amino acids so the medium resembles the protein composition of cultured mammalian cells
Preparation Note
Formulated to contain 9.3 grams of powder per liter of medium.
Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate.
