Minimum Essential Medium Eagle

General description
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.[1]This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Often used in cytogenetic analysis when supplemented with fetal bovine serum (FBS).
Application
Minimum Essential Medium Eagle has been used for the isolation of mesenchymal stem cell, adipose-derived stem cell,[2] and osteoblast.[3] Minimum Essential Medium Eagle is used for primary osteoblast cultures[4] and for isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.[5] Biochem/physiol Actions
MEM usually autoclaved with 0.5% bactopeptone at 15 psi for 20 min, cooled and then neutralized with NaHCO3, consistently supported good cell growth. A similar result was obtained when the MEM and bactopeptone were autoclaved separately. The cells grow, initially, as a monolayer and subsequently become a stationary suspension.[6] Preparation Note
Supplement with 0.292 g/L L-glutamine