NT-3 human

General description
NTF3 (neurotrophin 3) is a neurotrophic factor belonging to the NGF (nerve growth factor) family.[1] This protein is composed of 257 amino acids, with a prepro region of 138 amino acids and the mature sequence of 119 residues.[2] Biochem/physiol Actions
NT3 functions on two structurally unrelated receptor types- the p75 neurotrophin receptor (p75NTR) and the receptor tyrosine kinase (TRK). It promotes the metastatic invasion of brain by breast cancer cells, by promoting the EMT (epithelial-mesenchymal transition) transition of breast cancer cells and suppressing the immune response in brain.[1] This protein is responsible for the indction and survival of neurite outgrowth from a subset of neural crest (NC) and placode-derived neurons.[3] Recombinant human NT-3 is a noncovalently linked homodimer, of two 13.6kDa polypeptide monomers (240 total amino acid residues).
Physical form
Lyophilized with no additives.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.