NT-3 human

General description
NT-3 (neurotrophin 3) is a neurotrophic factor structurally related to β-NGF (nerve growth factor), BDNF (brain-derived neurotrophic factor), and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. NT-3 is expressed by neurons of the central nervous systems and can signal through the trk receptors.[1] Recombinant human NT-3 is a noncovalently linked homodimer, of two 13.6kDa polypeptide monomers (240 total amino acid residues). The amino acid sequences of human, murine and rat NT-3 are identical.
Biochem/physiol Actions
NT-3 (neurotrophin 3) promotes the growth and survival of nerve and glial cells.[1] It is associated with neuroprotection and polymorphisms in the gene are associated with risk to Alzheimer′s disease.[2] It is also linked with attention-deficit/hyperactivity disorder (ADHD) and mood disorders.[3] NT-3 enhances the metastatic proliferation of breast cancer cells in the brain.[4] Physical form
Lyophilized with no additives.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
MYAEHKSHRG EYSVCDSESL WVTDKSSAID IRGHQVTVLG EIKTGNSPVK QYFYETRCKE ARPVKNGCRG IDDKHWNSQC KTSQTYVRAL TSENNKLVGW RWIRIDTSCV CALSRKIGRT