Platelet-Derived Growth Factor-BB human

General description
Platelet-derived growth factor (PDGF) is expressed as homo and heterodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.[1] The PDGF gene is highly conserved mapped to human chromosome 7p22.3.[2][3] The active form of Pdgf exists as disulfide-linked dimers. It consists of cystine-knot-fold growth factor domain, which is highly conserved, except for the N-terminal prodomain, which shows variation in length among the isoforms.[4] Application
Platelet-Derived Growth Factor-BB human has been used :
As a growth supplement in proximal tubule and glomerular explant cell culture[5] In three-dimensional scaffold preparation[6] To study the effect of brivanib on growth factor signaling pathways[7] In transwell migration assay[8] Biochem/physiol Actions
Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes.[9] PDGF significantly activates chemotaxis of neutrophils, monocytes, and fibroblasts.[10][9] It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair.[11] PDGF-BB isomer is found to be critical in angiogenesis and is highly expressed in tumor, mediating oncogenic pericyte recruitment.[1] PDGF-BB is an important regulator of bone homeostasis, repair and regeneration.[12] Physical form
Lyophilized from a 0.2 μm filtered solution in 30 mM acetic acid.
Analysis Note
The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.
Other Notes
Dimer of the B chain of human PDGF.