English
General description
QCM ECMatrix Cell Invasion Assay involves invaded cells at the bottom of the insert membrane and can be obtained by treating the membrane with cell detachment buffer. The cells are lysed and detected by the patented CyQuant GR dye. This dye is a green-fluorescent dye and binds well with cellular nucleic acids to emit a strong fluorescence. This assay kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.
Application
QCM ECMatrix Cell Invasion Assay, 96-well (8 μm), fluorimetric has been used in the cell invasion assay of:
human cervical carcinoma[1]
osteosarcoma[1]
breast cancer cells[1]
colorectal cancer cells[2]
Ideal for screening pharmacological agents, evaluating MMPs or proteases responsible for cell invasion, and gene function analysis in transfected cells
Features and Benefits
The assay does not require cell labeling, scraping, washing, or counting
Suitable for large-scale screening and quantitative comparison of multiple samples due to homogenous fluorescence detection and 96 well insert
Enables a quick and efficient system for the quantitative determination of several factors for cell invasion
Other Notes
Sterile 96-well Cell Invasion Plate Assembly: (Part No. 90200) One 96-well feeder tray, and one 96-well Cell Invasion Chamber plate with ECMatrixTM -coated inserts.
96-well Cell Culture Tray: (Part No. 90129) One 96-well feeder tray.
Cell Detachment Solution: (Part No. 90131) One bottle – 16 mL.
4X Cell Lysis Buffer: (Part No. 90130) One bottle – 16 mL.
CyQuant GR Dye: (Part No. 90132) One vial – 75 μL
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
