EnglishGeneral description
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
Preparation Note
Formulated to contain 10.3 grams of powder per liter of medium.
Supplement with 0.3 L-glutamine, 2.0 g/L sodium bicarbonate.
Other Notes
Modified for autoclaving
Without L-glutamine and sodium bicarbonate.
Legal Information
Auto-Mod is a trademark of Sigma-Aldrich Co. LLC
RPMI-1640 Medium
AutoMod™, without L-glutamine and sodium bicarbonate, powder, suitable for cell culture
Code: R7755
- Hãng sản xuất: Merck
- Thương hiệu: SAFC
- Hãng sản xuất: Merck
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Sản phẩm tương tự
Accutase® solution
sterile-filtered, suitable for cell culture
Code: A6964
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Bovine Serum Albumin
lyophilized powder, ≥96% (agarose gel electrophoresis)
Code: A2153
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Dimethyl sulfoxide
sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Code: D2438
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Minimum Essential Medium Eagle
With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Code: M4655
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Dimethyl sulfoxide
Hybri-Max™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Code: D2650
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
RPMI-1640 Medium
With ʟ-glutamine, sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Code: R8758100 mL R8758-100ML
500 mL R8758-500ML
1 L R8758-1L
6 x 500 mL R8758-6X500ML
6 x 1 L R8758-6X1L
24 x 500 mL R8758-24X500ML
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Bovine Serum Albumin
heat shock fraction, pH 7, ≥98%
Code: A9647
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Fetal Bovine Serum
USDA approved, sterile-filtered, suitable for cell culture
Code: F0926
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Dimethyl sulfoxide
≥99.5% (GC), suitable for plant cell culture
Code: D4540
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Fetal Bovine Serum
non-USA origin, sterile-filtered, suitable for cell culture
Code: F7524
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
