Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor human

General description
Stromal Cell-Derived Factor 1α (SDF-1α) or pre-B Cell Growth Stimulation Factor (PBSF) is an 8 kDa chemokine peptide.[1] SDF-1 was identified using signal sequence trap cloning. With this method, cDNAs have been cloned using mouse and human stromal cell lines.[2] This cytokine is widely accepted as a CXC chemokine of the intercrine-macrophage inflammatory protein superfamily. The gene has been mapped to chromosome 10q and is encoded by 4 exons.[1] Expression of the human SDF1 gene is abundant in the pancreas, spleen, ovary, and small intestine.[3] The presence of a GC-rich sequence and the lack of a TATA box in the 5∅-flanking region of the gene are consistent with ubiquitous expression.[1] Biochem/physiol Actions
SDF-1α functions as a pre-B cell growth factor in the presence of IL-7 (interleukin-7) and a potent chemoattractant for T-lymphocytes and monocytes.[4] It has also been found to be a ligand for CXCR4 (chemokine receptor type 4) or the orphan receptor LESTR/fusin (putative chemokine receptor). By signaling through the receptor, SDF1 may serve as an inhibitor of HIV-1 (human immunodeficiency virus), which utilizes the LESTR/fusin receptor as a point of entry.[5] The SDF-1/CXCR4 signalling pathway is associated with cancer cell proliferation, migration and survival.[2] Overexpression of SDF1 is observed in breast carcinoma, ovarian carcinoma and papillary thyroid carcinoma. It enhances angiogenesis in the ischemic tissue. SDF1 offers CXCR4 mediated neuroprotection during post ischemic stroke.[6][7] Physical form
This product is lyophilized from a 0.2 μm-filtered solution containing 10 μg SDF-1α and 0.5 mg bovine serum albumin in PBS.
Preparation Note
Reconstitute the contents of the vial using sterile buffer or medium containing a minimum of 0.1% BSA or HSA to a stock concentration of ≥10 μg/ml. Additional filtration of the stock solution is not recommended as this may result in loss of product due to adsorption onto the filter membrane.