WNT-1 human

General description
Wnt family member 1 (Wnt-1) is a secreted protein that signals through the Frizzled family of cell surface receptors.[1] Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. The gene encoding this protein is localized on human chromosome 12q13.12.[2] Recombinant human Wnt-1 is a 38.4kDa, non-glycosylated protein containing 343 amino acid residues.
Application
Wnt family member 1 (Wnt-1) has been used in the differentiation medium for human neural progenitor cells (hNP1).[3] Biochem/physiol Actions
Wnt family member 1 (Wnt-1) and is required for normal embryonic development.[1] Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over 50 genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, β-catenine.[2][1] Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self-renewal in the absence of significant differentiation.[4] Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers.[1] Physical form
Sterile filtered and lyophilized with no additives.
Preparation Note
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in water to a concentration of 0.1-1.0 μg/μL. The solution can then be diluted into other aqueous buffers.