Wnt3A, human

General description
WNT3A (Wnt family member 3A) gene is mapped to human chromosome 1q42.13.[1] Wnt3A gene is a member of the Wnt gene family that consists of structurally linked genes that encode secreted signaling proteins.
Biochem/physiol Actions
WNT3A (Wnt family member 3A) functions mainly to induce canonical Wnt signaling pathway.[2] Wnt signaling pathway is essential for gastrulation, organogenesis, tissue development, homeostasis and regeneration. Congenital defects, oncogenic transformation and many more disorders results due to abnormal Wnt signaling pathway.[3] During the differentiation of human neuronal progenitor cells, WNT3A (Wnt family member 3A) activates calmodulin-dependent kinase II and increases free calcium in the cytosol via Wnt/Ca2+ signaling pathway.[4] WNT3A is an oncogene and is upregulated in many types of cancer. It serves as a prognostic marker in diagnosing hepatocellular carcinoma.[1][5] It also promotes metastasis of malignant tumors, including gastric cancer, colorectal cancer, non-small cell lung cancer and breast cancer.[6] Physical form
Lyophilized from a 0.2 μm filtered solution of 1X PBS pH 7.4 + 500mM NaCl + 1% CHAPS.
Preparation Note
It is recommended to reconstitute the protein to ≤100μg/mL in sterile 1X PBS containing 0.1% endotoxin-free HSA.
Upon reconstitution, the cytokine can be stored at 4°C for short term or at -20°C to -80°C in aliquots for long term. Avoid repeated freeze-thaw cycles.
Analysis Note
The activity was determined by dose dependent Alkaline Phosphatase production in MC3T3 E1 mouse preosteoblast cells and stabilization of beta catenin in mouse L929 cells.