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General description
Normal carrier sera from healthy, non-immunized mice.
Application
ELISA (see comments)
Flow Cytometry (see comments)
Immunoblotting (see comments)
Immunofluorescence (see comments)
Immunohistochemistry (see comments)
Immunoprecipitation (see comments)
Physical form
Lyophilized serum. Lyophilized from 250 mM sodium chloride, 10 mM sodium phosphate, pH 7.6.
Preparation Note
Reconstitute the lypohilized serum in 1 ml of sterile distilled water with occasional gentle mixing for at least 2 h at 4°C. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
Other Notes
Useful as a blocking agent in immunohistochemical procedures or as a negative control for specific mouse antibodies. For use as a negative control in any application, perform experiments in parallel using primary antibody or normal serum at the same concentration. A signal obtained with the normal serum indicates a nonspecific interaction; any additional signal with the primary antibody indicates a specific antibody-antigen interaction. This antibody has also been reported to work for ELISA, flow cytometry, immunoblotting, immunofluorescence, immunohistochemistry, and immunoprecipitation.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
