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General description
Human serum albumin (HSA) is a monomeric, globular, and α-helical protein that constitutes a major part of human blood plasma proteins. This single-chain polypeptide protein contains 585 amino acid residues and 17 internal disulfide bridges and one free cysteine.
Human serum albumin undergoes three different post-translational modifications: oxidation, glycation, and S-nitrosylation. Modifications usually occur on the surface of the globular protein, and do not significantly affect conformation. However, modification strongly affects binding of fatty acids and drug molecules.
Application
Albumin solution human has been used as a supplement in RPMI 1640 media during polymorphonuclear cell (PMN) incubation. It has also been used as a culture media for the differentiation and maturation of monocyte-derived dendritic cells.
Biochem/physiol Actions
Human serum albumin (HSA) plays a vital role in the regulation of the colloidal osmotic pressure of blood. It also aids in the maintenance of metal ion homeostasis, including the transport and storage of transition metals. The high binding capacity of HSA with a wide range of ions and molecules makes it an effective molecular cargo and nano vehicle used in biophysical, clinical, and industrial fields. Human and bovine albumins contain 16% nitrogen and are often used as standards in protein calibration studies. It is used as a blocking agent in Western blots or enzyme-linked immunosorbent assay (ELISA) applications. Globulin-free albumins are suitable for use in applications where no other proteins should be present (e.g., electrophoresis).
Features and Benefits
Easily crystallized and contain an excess of acidic amino acids.
Serum and plasma albumin is carbohydrate-free and comprises 55-62% of the protein present
Due to its high charge to mass ratio albumin binds water, Ca2+, Na+, K+, fatty acids, bilirubin, hormones, and drugs.
The free hydrophobic region of fatty acid-free albumins helps to solubilize lipids in tissue culture
Other Notes
View more information on human serum albumin.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
