English
General description
Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) – and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
Biochem/physiol Actions
Depositor
Klinikum der Universität zu Köln
Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Bad Oeynhausen, Germany
Tissue collection date: Aug. 5, 2009
Passage number reprogrammed: P3
Reprogramming method
Vector type: Integrating
Vector: Transposon
Transposon: Sleeping beauty
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the used vector excisable?: yes
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Yes
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Vector Free Reprogramming
Types of factor(s) used: miRNA
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG(+)POU5F1 (OCT-4)(+)SSEA-4(+)TRA 1-80(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:B-TUBULIN(-)FOXG1(+)HES5(+)NEUROD1(+)PAX6(+)Sox1(+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:CXCR4(+)FOXA2(-)GATA6 (-)GSC(+)PITX1(+)SOX17 (-)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:BMP4 (+)DCN(+)GATA4(+)HAND1(+)PDGF(-)PECAM(+)VIMENTIN(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P21
Cell line karyotype: Normal male chromosome complement and banding pattern.
Karyotyping method: G-Banding
Genotyping
Genome-wide analysis:
Molecular karyotyping using OmniExpress Exome Chip
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Preparation Note
Medium: Essential E8®
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°C
Other Notes
Additional Cell Line Info
EBiSC Access Use Agreement (EAUA)
Cell Line Information Pack (CLIP)
Note: EAUA and CLIP must be completed before order fulfillment
Legal Information
E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
