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General description
Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.
Chromolith® Si columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å). Chromolith® Si columns fall under the USP L3 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® Si columns are suitable for LC-MS applications.
The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for separations of complex samples on any HPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® Si 5-4.6 guard cartridges (3 pieces) [1.52011] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Application
Improved separation and quantification of neutral and polar lipid classes: This study exemplifies the application of Chromolith® Si in environmental sample analysis, offering enhanced separation and quantification capabilities crucial for monitoring marine lipids (Graeve M et al., 2009).
HPLC separation of some purine and pyrimidine derivatives: Highlighting the precision of Chromolith® Si in pharmaceutical research, this study focuses on the high-performance liquid chromatography separation of nucleobase derivatives, providing essential data for drug development (Kazoka H, 2007).
Analysis Note
Pressure: ≤ 15 bar
Performance (2-nitroanisol)
Theoretical Plates (N/m) (2-nitroanisol): ≥ 80000
Capacity factor (2-nitroanisol): 1.4 – 1.9
Tailing (USP): 0.90 – 1.45 %
Selectivity
separation factor (2-nitroanisol/nitrobenzene): 3.45 – 3.95
Legal Information
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
