EnglishGeneral description
This DMEM-Hi glucose medium differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.
Application
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name “DMEM/High”.
Legal Information
StableCell is a trademark of Sigma-Aldrich Co. LLC
DMEM – high glucose
StableCell™, with stable glutamine, sodium pyruvate and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Code: D0822
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
- Hãng sản xuất: Merck
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Sản phẩm tương tự
D-(+)-Glucose
powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%
Code: G7021
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Dimethyl sulfoxide
Hybri-Max™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Code: D2650
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Millipore® Stericup® Quick Release Vacuum Filtration System
pore size 0.22 μm, polyethersulfone membrane, filter capacity 500 mL, pack of 12 ea
Code: S2GPU05RE
- Hãng sản xuất: Merck
- Thương hiệu: Millipore
Poly-ʟ-Ornithine
synthetic, mol wt 30,000-70,000, liquid, 0.01%, suitable for cell culture
Code: P4957
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Fetal Bovine Serum
USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma
Code: F2442
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Millipore® Steriflip® Vacuum Tube Top Filter
pore size 0.22 μm, polyethersulfone membrane, funnel capacity 50 mL, pack of 25 ea
Code: SCGP00525
- Hãng sản xuất: Merck
- Thương hiệu: Millipore
RPMI-1640 Medium
With ʟ-glutamine, sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Code: R8758100 mL R8758-100ML
500 mL R8758-500ML
1 L R8758-1L
6 x 500 mL R8758-6X500ML
6 x 1 L R8758-6X1L
24 x 500 mL R8758-24X500ML
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Dimethyl sulfoxide
≥99.5% (GC), suitable for plant cell culture
Code: D4540
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Insulin solution human
sterile-filtered, BioXtra, suitable for cell culture
Code: I9278
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
Poly-ʟ-Lysine Hydrobromide
synthetic, liquid, 0.01%, suitable for cell culture
Code: P4707
- Hãng sản xuất: Merck
- Thương hiệu: Sigma-Aldrich
