English
General description
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains a higher concentration of amino acids, vitamins, and additional supplementary components. The original DMEM formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. This modification has been shown to improve the stability of the medium.
Application
Dulbecco′s Modified Eagle′s Medium – low glucose has been used to culture:
human osteosarcoma cells and HeLa cells[1]
primary human mesenchymal stromal cells (hBMSCs)[2]
human embryonic kidney cell lines[3]
Preparation Note
Supplement with 0.584 g/L glutamine.
