English
General description
Histopaque®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm3.[1] This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell-mediated lympholysis and human lymphocyte antigen (HLA)2 typing. It may also be employed as the initial isolation step before the enumeration of T-, B-, and null lymphocytes. It is used to create a density medium for the purification of lymphocytes and other mononuclear cells.
Application
Histopaque®-1077 Hybri-Max™ has been used to isolate:
peripheral blood mononuclear cells (PBMCs) from heparinized blood by gradient centrifugation[2]
immune cells (lymphocytes) from peripheral blood[3]
PBMCs from blood[4]
Legal Information
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
