Complement C5-Depleted Serum, Human

General description
Human serum depleted of complement component C5 by affinity chromatography.
Human serum that has been depleted of complement component C5 by affinity chromatography. Intended for the detection and quantitation of hemolytically active C5 complement component. The classical and alternative pathways are intact except for the specifically depleted component. Recommended assay volume: ≤60 µl
Biochem/physiol Actions
Complement C5-depleted serum from humans produced by affinity chromatography aids the development of the complement cascade up to the desired convertase level. It eliminates the usual time-consuming, expensive, and stepwise formation of enzymatic complexes from purified products. Whole serum usage reduces the risk of protein inactivation during the process. It also promotes better physiological conditions.[1] Complement C5-Depleted Serum is intended for the detection and quantitation of the hemolytically active C5 complement component. Creating complement convertases on erythrocytes enhances a better readout of the assay by spectrophotometric quantification of released hemoglobin.[1] Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 145 mM NaCl, 10 mM sodium phosphate, pH 7.3.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Prepared from plasma of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)