English
General description
Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. Suitable for growth of a wide range of cell types in low serum conditions. Absence of phenol red makes this a preferred medium for clonal and stem cell culture.
Application
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture:
bovine endometrial epithelial cells[1]
cancer stem cells[2]
breast cancer cell line[3]
Preparation Note
Supplement with 0.365 g/L L-glutamine
