HGF human

General description
HGF (hepatocyte growth factor) is a multifunctional heterodimeric mitogen, with a relative molecular mass (Mr) of 82,000, consisting of a large α-subunit of Mr 69,000 and a small β -subunit of Mr 34,000. The pre-pro precursor protein is composed of 728 residues. HGF is produced by mesenchymal cells.[1][2] The hepatocyte growth factor (HGF) gene is located on human chromosome 7q21.1. It has 18 exons, 17 introns and spans approximately 70 kb. Active HGF is made up of a heavy chain and a light chain, connected covalently with the help of a disulfide bridge.[3] Biochem/physiol Actions
HGF (hepatocyte growth factor) functions as a ligand for tyrosine kinase receptor Met. Through interaction with Met receptor, it modulates mitogenesis, motility, and morphogenesis in a cell-specific manner. It is implicated in the growth and scattering of specific cell types, EMT (epithelial mesenchymal transition), formation of lumens and tubules, and angiogenesis. HGF-Met is also involved in neural induction, hepatic regeneration, wound healing, and normal fetal development.[1] In primary culture, HGF acts as a strong mitogen for mature parenchymal hepatocytes, and is thought to be a hepatotrophic factor which induces liver regeneration post-partial hepatectomy and liver injury.[2] In HER2-positive patients with metastatic gastric cancer, the serum level of this protein is linked with the outcome and resistance to trastuzumab therapy.[4] HGF exhibits mitogenic, motogenic and morphogenic effects in various tissues.[3] Physical form
Lyophilized in solution of 10 mM Tris-HCl, pH 7.4, with 1 M NaCl.
Preparation Note
Centrifuge the vial prior to opening.
Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.